Insertion junction: CLIP2.005730_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.005730
Chromosome:chromosome 1
Location:7398649
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g052100 PRPL18 (1 of 1) K02881 - large subunit ribosomal protein L18 (RP-L18, MRPL18, rplR); Chloroplast ribosomal protein L18 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCCAACGCGCGCGTACTGCTTGCATCAAGCCGCCGCTGCGTCCCTAGC
Internal bar code:CTAGGATGTAAATTCGCTTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:5945
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGAGTCGGACGGGAAAG
Suggested primer 2:GGCCCTGAACGACTGAATGA