Insertion junction: CLIP2.005751_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.005751
Chromosome:chromosome 1
Location:2904163
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g018075 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTTCCTTCTTGTCGCAGCAGGTGCCTGGTCCAGTACGTGGTTGATTGC
Internal bar code:AATGAATCCCACCGGACAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1485
LEAP-Seq percent confirming:97.7273
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCATCTGCTCTTGCTC
Suggested primer 2:CTACCCCAAACTCCGTCACC