Insertion junction: CLIP2.005763_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.005763
Chromosome:chromosome 14
Location:1382506
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616900 SULP2,SLP2 Chloroplast sulfate permease; (1 of 1) K02047 - sulfate transport system permease protein (cysW) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGCGGGGATAACACCCTCTGTCCCCATTGCGGCGTGTTGGCTAGTCTT
Internal bar code:GCCGGAGAGAGTAATACAAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1546
LEAP-Seq percent confirming:80.6452
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTGTACTCCTGCGTGGA
Suggested primer 2:TGCTTTGTGACGTTTCAGCG