Insertion junction: CLIP2.005763_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005763
Chromosome:chromosome 14
Location:1382510
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616900 SULP2,SLP2 Chloroplast sulfate permease; (1 of 1) K02047 - sulfate transport system permease protein (cysW) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCGGTGCATTGCAGACCTGGACACCCGCACGCGCCGAAGCACATGCT
Internal bar code:TAAGAAACAAGGTTAATCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2996
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTTGTGACGTTTCAGCG
Suggested primer 2:GTGTTGTACTCCTGCGTGGA