Insertion junction: CLIP2.005774_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005774
Chromosome:chromosome 6
Location:3928295
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278195 CTI,CTI1 Carboxyltransferase interactor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATTGAGAAGATTGCTCAGCTCAACGCCTCCATCGACGAGGTGGCCGCG
Internal bar code:GGCTGGTACATATCTGCGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2741
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTACTCGCCCTATGTCAC
Suggested primer 2:GTGTCGTCAGTGCACTGGTA