| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005799 |
| Chromosome: | chromosome 17 |
| Location: | 967374 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703000 | (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAACACCACCCCCAACACCAACACCAACACCATCCCCAACACCAGCG |
| Internal bar code: | GCTGGACAGTTCACGACGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2961 |
| LEAP-Seq percent confirming: | 68.254 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGATGGTGGGAATGCT |
| Suggested primer 2: | TCCCGAAAACCCAAAACCCA |