| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005801 |
| Chromosome: | chromosome 14 |
| Location: | 2535537 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624650 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGGGTTGCACTACTTTTTGGTACGTGGCTTGCTGGCTTCATTACCA |
| Internal bar code: | GAATTAGACGTTTGGGTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3823 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGTGCACGCTGAACATA |
| Suggested primer 2: | GGGCCATTTCCTCATGGTGA |