Insertion junction: CLIP2.005804_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.005804
Chromosome:chromosome 2
Location:1492365
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g083950 PSRP3,cS23 Plastid-specific ribosomal protein 3; (1 of 1) K19032 - 30S ribosomal protein 3 (PSRP3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTTAGGAAGGGTCGGCGCTTGGGCACGCTTGCAGGCCTAGGAAGACGC
Internal bar code:AAATTTTTTACTGCAGGCCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1676
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACACACCCTCCTGTTC
Suggested primer 2:TGGATGTGGATGACATGGGC