| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005813 |
| Chromosome: | chromosome 12 |
| Location: | 49552 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483600 | (1 of 1) PTHR24198//PTHR24198:SF93 - ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre12.g483650 | STL1 | (1 of 3) PTHR11584:SF316 - SERINE/THREONINE-PROTEIN KINASE STN7, CHLOROPLASTIC; Ser-Thr protein kinase, paralog of STT7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGCTATGAGCATTCGGCAACGCAATCTGCCGCCATCCTGCCGAGTGT |
| Internal bar code: | TTGACGGGCCGGTTGAATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1883 |
| LEAP-Seq percent confirming: | 96.1165 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTATTACGGACCGGGCTG |
| Suggested primer 2: | ATAGGTCCCCACTGGAGGTC |