| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.005815 |
| Chromosome: | chromosome 3 |
| Location: | 6561297 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194450 | CGL24 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCACAGATGGAGGTGGGGCAGGGGCTGTATGGGCCTTCCTAGTGACTG |
| Internal bar code: | GGAGCGGGTGTTCGGCTGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1224 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAAAAGGGGAGCTGGCC |
| Suggested primer 2: | TGCTCATTCAGTACCGCCAG |