Insertion junction: CLIP2.005816_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005816
Chromosome:chromosome 3
Location:6654757
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g195200 HLD1,CGL76 Conserved in the Green Lineage; (1 of 6) IPR000073//IPR000639//IPR029058 - Alpha/beta hydrolase fold-1 // Epoxide hydrolase-like // Alpha/Beta hydrolase fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCGTTCCCCGCATAATCTCCTGACACATCGCGCTCAACCTCCTGCCA
Internal bar code:GGCCACATCGCTTGTCAACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3951
LEAP-Seq percent confirming:85.4701
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTTCAGCACCATCTCCT
Suggested primer 2:GTGTGGGGAATGGAAGAGCA