Insertion junction: CLIP2.005823_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005823
Chromosome:chromosome 8
Location:852476
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361250 (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACGACGACGTCGGCGGGCCGCTGCCCGCCACCGAGTCCTCCGTCGACT
Internal bar code:AGAATATTAACCTGTGGACCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCACCTCACCACAATCC
Suggested primer 2:CCAGGCAGGAGTCTCAACTG