| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005823 |
| Chromosome: | chromosome 8 |
| Location: | 852483 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361250 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCCGCCGCTGCCGCCGCGCTTCTCTAGCCGCCGCCGCCGCCGCTTG |
| Internal bar code: | AGGAAGCTGTTCTATCTCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2686 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGCAGGAGTCTCAACTG |
| Suggested primer 2: | CTCCCACCTCACCACAATCC |