Insertion junction: CLIP2.005833_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005833
Chromosome:chromosome 12
Location:4997088
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g525100 SMC6B (1 of 2) PTHR19306//PTHR19306:SF6 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // PROTEIN C23H4.6, ISOFORM A-RELATED; Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGGGCCTCCAGCGCCCCACCCCGCTCACTGTTCCGTCTTTGCCGTCT
Internal bar code:ATCAATTATTCTATTCTGCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1044
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCATCGCAGTCCAGTCTT
Suggested primer 2:GCGATGTCAAGTGTGGATGC