| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005836 |
| Chromosome: | chromosome 6 |
| Location: | 7717781 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302650 | RRM5 | (1 of 2) 2.1.1.166 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase / Um2552 methyltransferase; Putative ribosomal RNA methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGAACCCTGGGGTGCTCACCTTACGCCGCGTCCCGCACACCTGCA |
| Internal bar code: | CCAACTTAATGGTTTAGTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 69.5652 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCACCGTGTCAAAACC |
| Suggested primer 2: | CGTCGGTACCAGTATGTCGG |