| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005852 |
| Chromosome: | chromosome 9 |
| Location: | 5353151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407801 | AKC1 | (1 of 1) PTHR10566//PTHR10566:SF41 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // SUBFAMILY NOT NAMED; ABC1/COQ8 ser/thr kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGCGCAAGCCAGCGACCATCGCATTGGAAACCACAGCTATTTATTA |
| Internal bar code: | CATCGGGCGTTAAGTTGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGGGTTGGTTTCATTTT |
| Suggested primer 2: | GGCCAGCACATGAAAAGCAA |