| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005854 |
| Chromosome: | chromosome 12 |
| Location: | 1884815 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512050 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTCCTGGTCCTGGTCACGCGCAGATTGAGTACCTGCTGTGCTACGC |
| Internal bar code: | ACCAGTTTGGGGGGAGACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3205 |
| LEAP-Seq percent confirming: | 64.1026 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTGATGATGTTCGGCCA |
| Suggested primer 2: | TCATGGTGCTTCTGCGGAAT |