Insertion junction: CLIP2.005855_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005855
Chromosome:chromosome 1
Location:877290
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g005001 CPLD9 Conserved in the Plant Lineage and Diatoms intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAACGCGCGTACCGTAAGACGCAAACGCGCCCATGCCGAGCTGTGCC
Internal bar code:ACTTGTCTTTTCATTTTCATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1550
LEAP-Seq percent confirming:45.5882
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTATACCCTGCGCAACG
Suggested primer 2:TAGACTCAGCTGTCTCCCCC