Insertion junction: CLIP2.005867_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005867
Chromosome:chromosome 12
Location:4174464
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g517700 NYC1,SDR21 (1 of 2) K13606 - chlorophyll(ide) b reductase (NOL, NYC1); Chlorophyll b reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCACGCCTGCATAACGTTTCCCTTCCACCCCGCCACAGGCGCCACCAA
Internal bar code:CGCAGAGACATCTCCAAAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1283
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATTCCCACAACACACAC
Suggested primer 2:ACCTCCATACCCTACGCCTT