| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005869 |
| Chromosome: | chromosome 16 |
| Location: | 4819003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687450 | CPLD54 | (1 of 1) PTHR16254//PTHR16254:SF6 - POTASSIUM/PROTON ANTIPORTER-RELATED // K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC; potassium ion transmembrane transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCTGTTCCTGCTGTTTGAGATGGGGCTTGAGCTGTCTCTGGACCGT |
| Internal bar code: | TAGTAGCTTGACAAATTTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGGAAGATGGTCCCGAT |
| Suggested primer 2: | GTGGTGTTGAAGCAGCTTGG |