| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005885 |
| Chromosome: | chromosome 6 |
| Location: | 1890155 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTCGGAGGAAGGGCAGTAGAAGCCAGCTTGGTCCTGTGAGAACGGCG |
| Internal bar code: | TGGTTCATGGTCCTCAGCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3436 |
| LEAP-Seq percent confirming: | 88.8199 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 161 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCTCACCCAAAAGGCAA |
| Suggested primer 2: | CACCTGGCCTTAAGTGGAGG |