| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005886 |
| Chromosome: | chromosome 3 |
| Location: | 4112206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172500 | PTO2,PTOX2 | (1 of 2) K17893 - ubiquinol oxidase (AOX1, AOX2); Plastid terminal oxidase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCGCGCGCCAGTACTGTGTTGGGGCTGCACGCTTTGTGCCTCGCGG |
| Internal bar code: | CCGTTCGGAGAGGCCACAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 3.125 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGGCGTACTAGGACATT |
| Suggested primer 2: | GGGGGAGGAAAAGGCTACAC |