Insertion junction: CLIP2.005903_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.005903
Chromosome:chromosome 6
Location:1609910
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261500 CPLD58 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR11260:SF136 - THIOREDOXIN FAMILY PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGTCAGGGACACGTTGTACCCCAGCACCAGGCCGCCGCTGCCCAGGC
Internal bar code:GCCCAACAGCACATCGAACTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2450
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGCACACGTGTCTCTCAC
Suggested primer 2:GTAACCACACACGCACACAC