| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005905 |
| Chromosome: | chromosome 6 |
| Location: | 1153015 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257950 | AST4 | (1 of 1) K14455 - aspartate aminotransferase, mitochondrial (GOT2); aspartate aminotransferase | 3'UTR |
| Cre06.g258000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGTGGTCTCCAGCAAGGTTGGGACTCTGTGTAACGCAATACCTCCTG |
| Internal bar code: | GCATGCGTATTTTATATGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2078 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCCGTGTGTTCTACTGT |
| Suggested primer 2: | TCTCCACCGACATGTGTGTG |