| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005911 |
| Chromosome: | chromosome 9 |
| Location: | 576807 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748397 | CGL143 | predicted protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGTACCGGTAGTCAGTCAGTCATTCAGTCCGGCTCGGCCACGGGTG |
| Internal bar code: | GGATTGATCGGGCATAATTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTACGGCGTTTCCTGCT |
| Suggested primer 2: | GGTCAGGGGACAGGATAGGT |