| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005924 |
| Chromosome: | chromosome 6 |
| Location: | 811910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255250 | putative splicing factor | 5'UTR | |
| Cre06.g255300 | CGLD28 | Conserved in the Green Lineage and Diatoms; (1 of 1) IPR000104//IPR025461 - Antifreeze protein, type I // Protein of unknown function DUF4281 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCCGAATGAATAGGCCCCGGGTGGTGGGTGTGGTGAGTGTCTGGTC |
| Internal bar code: | CCCCAGGGAAACGTTTTTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1327 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCCAGCTTGTACACGTC |
| Suggested primer 2: | TGCTTCTTCTTCGGACCCAC |