Insertion junction: CLIP2.005926_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005926
Chromosome:chromosome 10
Location:1731730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430150 REP27,LPA1 (1 of 1) PF11998//PF13414 - Protein of unknown function (DUF3493) (DUF3493) // TPR repeat (TPR_11); Low Photosystem II Accumulation 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTAAAACCTTGAAAGCGCCCACCCGCTGGACTCCCACTCTGGACGCCCT
Internal bar code:ATTGATTACACATTCAGTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2271
LEAP-Seq percent confirming:90.3226
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:124

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTGACTTAGCCTTGGG
Suggested primer 2:TGCCCCAGATCTCTCAGTCA