| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005980 |
| Chromosome: | chromosome 2 |
| Location: | 7828178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146300 | (1 of 2) PTHR10027 - CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGATGATGCGGCGGCCCCCCTGTGTCTCTTGTTGCTGACCGGCATG |
| Internal bar code: | GAGCCAACAGGATTCTAGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3621 |
| LEAP-Seq percent confirming: | 89.5349 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCAGAACCTGCTGAAGTA |
| Suggested primer 2: | AAAGCTTGAGCCGCAACAAG |