| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005987 |
| Chromosome: | chromosome 3 |
| Location: | 8796169 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206929 | RSE4,EGY1 | (1 of 1) PTHR31412:SF0 - ZINC METALLOPROTEASE EGY1, CHLOROPLASTIC-RELATED; membrane associated metalloprotease involved in chloroplast development | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTATCCTCGGACGCGGCGCGCGCAGCGTCGTCCACGCCCGCCTCCACC |
| Internal bar code: | GCGATCCTCCTAAATGCGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGAATGCCTGGAGAAATG |
| Suggested primer 2: | AACGAAAGCTCCATCCGTGT |