| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006003 |
| Chromosome: | chromosome 12 |
| Location: | 8723977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGACCCATGTTCATTCCACGGTGGACTGGATTATGGCAAACTTGGCA |
| Internal bar code: | CACTTATGAAGGCAGGGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1800 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGGCATGTTGTGTTTGT |
| Suggested primer 2: | CTGAGCTTGCCAACCCAAAC |