| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006036 |
| Chromosome: | chromosome 12 |
| Location: | 5580630 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531050 | RAA3,OPR67 | psaA mRNA trans-splicing factor; (1 of 45) IPR013584 - RAP domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCCCTTACAGCTTGACCACACTTCCGACAAGCCAGCCAAACAATAT |
| Internal bar code: | GCAATCTCTACGGGGCGTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6974 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTAGCCCTGGATCTGTG |
| Suggested primer 2: | CGCTGTAGGTAGCTCCATGG |