Insertion junction: CLIP2.006069_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006069
Chromosome:chromosome 12
Location:3582863
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g496150 (1 of 1) PTHR20953//PTHR20953:SF4 - KINASE-RELATED // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGATGGTACTGTCGTTGTTCAAGACGACCTCGACCAGCTTCTAACGGTG
Internal bar code:TCCTTCGAAGTATTTGCTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:609
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACAACCCTAACTGCCGC
Suggested primer 2:TAGGCACCGACGACCTCTTA