| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.006073 |
| Chromosome: | chromosome 13 |
| Location: | 4586250 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603500 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCGACAGATTTGTGTGTCATGGGTCATGGCGGATTGGCGGTGCCAC |
| Internal bar code: | AGTGTGGATGCTTCGCTCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTTGCGGTAGTTGGGTG |
| Suggested primer 2: | ACAGCTTTCGCTTCCGAGAA |