Insertion junction: CLIP2.006096_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006096
Chromosome:chromosome 2
Location:4485315
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g106600 RPS19 Cytosolic 80S ribosomal protein S19; (1 of 1) K02966 - small subunit ribosomal protein S19e (RP-S19e, RPS19) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGTTATGTGTAACACGCACACGCAATGCTTTGGTTCACAACGTTCTC
Internal bar code:AATTGTTGATCTCTTACACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1003
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTTCTCGTCTGCTCGATA
Suggested primer 2:GGTTCGCCACATCATGAAGC