| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006100 |
| Chromosome: | chromosome 1 |
| Location: | 995024 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005813 | POB31 | Proteome of basal body 31 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCCGAAAAGCGCGAGGACGGTACGGCGCCCCCTCCCCCGGTTCGCT |
| Internal bar code: | ATGGCCTCGCAGTTGCTGTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 15.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGAGCCCATGTAACACAC |
| Suggested primer 2: | ACCTCTACCCCATCTGACCC |