| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006116 |
| Chromosome: | chromosome 10 |
| Location: | 3251568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442650 | (1 of 1) PTHR10887:SF366 - DNA-BINDING PROTEIN-RELATED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGAAGTATCATATAGGTCTGCTGGGGGTTGCGAGCAGCCATACTACC |
| Internal bar code: | GATAACAGAGTCTGCGGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2320 |
| LEAP-Seq percent confirming: | 10.7143 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTCATAACGTGTCGCGC |
| Suggested primer 2: | TGCAGCCCAACGACAATTTG |