Insertion junction: CLIP2.006118_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006118
Chromosome:chromosome 3
Location:5554237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185250 SSS2,SSII (1 of 1) IPR001296//IPR011835//IPR013534//IPR015421 - Glycosyl transferase, family 1 // Bacterial/plant glycogen synthase // Starch synthase, catalytic domain // Pyridoxal phosphate-dependent transferase, major region, subdomain 1; Soluble starch synthase II 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCGTAACGGTGCTAGCCCCCAGCTCGATGATAGAAGCGAAGGCTTGCA
Internal bar code:TAACGTTGAACTTCCCCGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3545
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCAGGAGCAGAGAAATGT
Suggested primer 2:AAGGACATGCAAGCGAGGAA