Insertion junction: CLIP2.006138_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006138
Chromosome:chromosome 1
Location:2336253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAGACGCTGAGCAAGTCAGCCTTAGCTATTGGAATAGTCAGCTATAG
Internal bar code:ACCAAATATCAATAGATGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1887
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTCAGTAGCACGCAAGA
Suggested primer 2:CAGGTGTGAAGTACGGCAGT