Insertion junction: CLIP2.006149_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006149
Chromosome:chromosome 7
Location:804603
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g318200 CGLD34 (1 of 2) IPR001214//IPR019734 - SET domain // Tetratricopeptide repeat; histone methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTGATGGTCATCTCCGACCCCCGCCACCCGCTGGTCGTCGGCACGGC
Internal bar code:CGTCAATGGACAAGGGAAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3217
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCTCTGTTGCTTGGGTA
Suggested primer 2:GTTTCCCCCAATGTTTCCGC