| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006150 |
| Chromosome: | chromosome 6 |
| Location: | 1919302 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264350 | PRPL13 | (1 of 1) PTHR11545//PTHR11545:SF7 - RIBOSOMAL PROTEIN L13 // 50S RIBOSOMAL PROTEIN L13, CHLOROPLASTIC; Chloroplast ribosomal protein L13 | 3'UTR |
| Cre06.g264400 | (1 of 1) PTHR13992:SF7 - SMRTER, ISOFORM G | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGAGGAAGGCGCCCGTCAACCATACCATATTCCTCAACCCAGTGCGAT |
| Internal bar code: | AAAATCAGGTAAGCCGTGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2271 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGTCGGGACACATTGA |
| Suggested primer 2: | CCGTGGGCGTTCTTTTTGAG |