Insertion junction: CLIP2.006156_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.006156
Chromosome:chromosome 16
Location:1454081
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652550 PRPL24 (1 of 1) PTHR12903:SF1 - 50S RIBOSOMAL PROTEIN L24, CHLOROPLASTIC; Chloroplast ribosomal protein L24 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGGCGAACCCGGACACGCCCCGCAACCCCCCCGCCCTCTGCCCGCGC
Internal bar code:GTCATGTCCCTTATCTCAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3372
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAACGAGACTGGGCAAA
Suggested primer 2:GCTTAAGCAGATGCACGAGC