Insertion junction: CLIP2.006162_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006162
Chromosome:chromosome 6
Location:5256615
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284250 LHCBM8 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGTGTGAACAACGCCTTCGCCTTCGCCACCAAGTTCACCCCCTCCGC
Internal bar code:TTATAGTGGTTTGTGTGCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3294
LEAP-Seq percent confirming:86.3636
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGAACATCTTGATGCACGCC
Suggested primer 2:CGTTGCGATATGGTGTGCTG