Insertion junction: CLIP2.006162_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.006162
Chromosome:chromosome 6
Location:5256636
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284250 LHCBM8 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTGGACGGGGCCCTTGCCGGTCACGATGGCCTGGACGAAGAAGCCGA
Internal bar code:TTATAGTGGTTTGTGTGCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1739
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGCGATATGGTGTGCTG
Suggested primer 2:GGAACATCTTGATGCACGCC