| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006181 |
| Chromosome: | chromosome 1 |
| Location: | 321027 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001750 | TIG1 | chloroplast trigger factor; (1 of 1) PTHR30560 - TRIGGER FACTOR CHAPERONE AND PEPTIDYL-PROLYL CIS/TRANS ISOMERASE | 5'UTR |
| Cre01.g001800 | MLCK1,FAP403 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; Flagellar Associated Protein 403 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAAACCAATGAAAGCACGGTGGCTGTGGCCGCATGCGAACTTCATGC |
| Internal bar code: | CGTGTACTTCGAAGGATAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACAGCAGGTTCACAACG |
| Suggested primer 2: | GAAAGGGGAGAGGGCAGAAC |