Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.006185 |
Chromosome: | chromosome 3 |
Location: | 3508365 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g167450 | (1 of 22) PF13640 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_3) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCCTGCCGGCGCCCCAGGCGGCGGAGCTGCGTGGCGTGTTCGACAGC |
Internal bar code: | CGGTTAAGGCTGACAAGGGTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1931 |
LEAP-Seq percent confirming: | 91.3043 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTAGCTCATCCAGATGGGGC |
Suggested primer 2: | TCGAGCTAAAGGGAGTCGGA |