| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006213 |
| Chromosome: | chromosome 3 |
| Location: | 2099416 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156500 | (1 of 1) IPR000104//IPR016084 - Antifreeze protein, type I // Haem oxygenase-like, multi-helical | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAACAATGCCGGTCCTCATCAGCAGCACCTGTAGGAAATACCCGGC |
| Internal bar code: | TTTTGGGTCTCGGTCAAGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3483 |
| LEAP-Seq percent confirming: | 75.7576 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGCACAAACACACCTCG |
| Suggested primer 2: | AAGCCATGTCACCACCTAGC |