| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006219 |
| Chromosome: | chromosome 1 |
| Location: | 5214981 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036400 | SDR3 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.313 - Sulfoacetaldehyde reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGAGCAGCCTGCCCACACCCCTCCACCCTCCACCCCGCCTCCTCCTC |
| Internal bar code: | TTAAGTATGTTAGTCGGTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAATCCGTGCTCCGAAGT |
| Suggested primer 2: | CATTACGGACATGCCTTGCG |