Insertion junction: CLIP2.006222_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006222
Chromosome:chromosome 6
Location:5367543
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285200 NDH1 Nucleoside diphosphate hydrolase; (1 of 1) 3.6.1.13//3.6.1.18 - ADP-ribose diphosphatase / ADPR-PPase // FAD diphosphatase / FAD pyrophosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGTGACTGCTGCCACTGCTGCCACTGCTGCTACTGCCGCTGCTGCTAC
Internal bar code:TGAGTCACCAGGGACTTAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1289
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCAGTGTGTCCATCACC
Suggested primer 2:TCGACAGCTGTTCCAAGTCC