| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.006228 |
| Chromosome: | chromosome 9 |
| Location: | 1604688 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398700 | CFA2,CPLD27 | (1 of 1) 2.1.1.140 - (S)-coclaurine-N-methyltransferase; coclaurine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGCTCCAGCGACTCCCGCGGCGAGCGGTAGAGGCAGGAGGAGTACT |
| Internal bar code: | ATATGGGAACGGACGTGAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 112 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTACGAGGTGTGTGTG |
| Suggested primer 2: | CTCTCCGTCAATGAACGCCT |