| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006236 |
| Chromosome: | chromosome 5 |
| Location: | 550412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244450 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR | |
| Cre05.g244500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACTGCGCATCCGCCGCATGTGCCGCGTCTGGCTGCGCTGCGGGGCCC |
| Internal bar code: | ATAATACTAAGTTGCGCAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1396 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGCAGACACAGAGACAC |
| Suggested primer 2: | TTGTGTGCGATCCGTTACCA |